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Mikrofluidik ermöglicht schnelle Analyse

Legionellen in Rekordzeit quantifizieren

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[1] D. J. Brenner, A. G. Steigerwalt, and J. E. McDade, “Classification of the Legionnaires’ disease bacterium: Legionella pneumophila, genus novum, species nova, of the family Legionellaceae, familia nova,” Ann. Intern. Med., vol. 90, no. 4, pp. 656–658, Apr. 1979.

[2] ECDC, “Legionnaires’ disease in Europe, 2010,” European Centre for Disease Prevention and Control, Stockholm, 2012.

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[3] T. Kistemann, “Hygienisch-mikrobiologische Risiken von Großgebäude-Wasserinstallationen,” Bonn, Germany, 2004.

[4] S. Völker, C. Schreiber, and T. Kistemann, “Drinking water quality in household supply infrastructure--A survey of the current situation in Germany,” Int. J. Hyg. Environ. Health, vol. 213, no. 3, pp. 204–209, Jun. 2010.

[5] “List of Legionnaires’ disease outbreaks,” Wikipedia, the free encyclopedia. 25-Jan-2014.

[6] Wikipedia, “Legionellose-Ausbruch in Warstein 2013,” Wikipedia. 10-Jan-2014.

[7] ISO, “ISO 11731. Water quality - detection and enumeration of Legionella,” International Organization for Standardization, Geneva, Switzerland, 2004.

[8] C. A. Boulanger and P. H. Edelstein, “Precision and accuracy of recovery of Legionella pneumophila from seeded tap water by filtration and centrifugation.,” Appl. Environ. Microbiol., vol. 61, no. 5, pp. 1805–1809, May 1995.

[9] R. H. Bentham, “Routine sampling and the control of Legionella spp. in cooling tower water systems.,” Curr. Microbiol., vol. 41, no. 4, pp. 271–275, Oct. 2000.

[10] C. Napoli, R. Iatta, F. Fasano, T. Marsico, and M. T. Montagna, “Variable bacterial load of Legionella spp. in a hospital water system,” Sci. Total Environ., vol. 408, no. 2, pp. 242–244, Dec. 2009.

[11] C. E. Lucas, T. H. Taylor Jr, and B. S. Fields, “Accuracy and precision of Legionella isolation by US laboratories in the ELITE program pilot study,” Water Res., vol. 45, no. 15, pp. 4428–4436, Oct. 2011.

[12] J. D. Oliver, “Recent findings on the viable but nonculturable state in pathogenic bacteria,” FEMS Microbiol. Rev., vol. 34, no. 4, pp. 415–425, Jul. 2010.

[13] S. Allegra et al., “Evaluation of an immunomagnetic separation assay in combination with cultivation to improve Legionella pneumophila serogroup 1 recovery from environmental samples,” J. Appl. Microbiol., Jan. 2011.

[14] S. Allegra, F. Berger, P. Berthelot, F. Grattard, B. Pozzetto, and S. Riffard, “Use of flow cytometry to monitor Legionella viability,” Appl. Environ. Microbiol., vol. 74, no. 24, pp. 7813–7816, Dec. 2008.

[15] B. Bendinger and J. Benölken, “Prexisnahe Untersuchungen zur Kontamination von Trinkwasser in halbtechnischen Trinkwasser-Installationen,” presented at the 24. Mühlheimer Wassertechnisches Seminar, Mühlheim, 19-May-2010.

[16] F. Hammes, M. Berney, Y. Wang, M. Vital, O. Köster, and T. Egli, “Flow-cytometric total bacterial cell counts as a descriptive microbiological parameter for drinking water treatment processes,” Water Res., vol. 42, no. 1–2, pp. 269–277, Jan. 2008.

[17] F. Hammes and T. Egli, “Cytometric methods for measuring bacteria in water: advantages, pitfalls and applications,” Anal. Bioanal. Chem., vol. 397, no. 3, pp. 1083–1095, Jun. 2010.

* *Dr. H.-A. Keserue, A.-K. Ehlert & Dr. D. Schaffhauser: rqmicro AG, 8952 Schlieren/Schweiz